Fig. 7
From: ALDH1A inhibition sensitizes colon cancer cells to chemotherapy
The tumor volume recordings from Balb/c nu/nu mice models for in vivo experiments. Tumors were induced by s.c. administration of 2.5 105 HT-29/eGFP cells transfected with ALDH1A1 siRNA or negative siRNA cells resuspended in 100 μl of serum-free DMEM. Animals (n = 8) were divided into two groups females (n = 4) and males (n = 4). The tumor volume was measured, duly and carefully, from 4th day post inoculation. The mice were screened for tumor growth and the tumor volume was calculated according to the formula: volume = length x width2/2