Fig. 3

ERK binds to STAT1 in ESCC. a. The interaction of endogenous ERK and STAT1 was investigated by using co-immunoprecipitation experiments in EC1 and KYSE150 cells treated with or without MG132. Co-immunoprecipitation was carried out using negative control IgG, anti-STAT1, or anti-ERK antibody. b. Co-localization of ERK and STAT1 in EC1 cells were examined by using confocal microscopy (scale bar, 5 μm). c. GFP-STAT1 (WT), GFP-STAT1 (Y701F), or GFP-STAT1 (S727A) were transfected into EC1 cells. Co-immunoprecipitation was performed using GFP antibody and immunoblotting was done using an anti-ERK antibody. Similar results were observed in three independent experiments. (E.V.: Empty vector)