Fig. 3

Upregulation of miR-29b-2-5p expression induces PDAC cells apoptosis and G1 phase cell cycle arrest. SW1990 and Capan-2 were transiently transfected with miR-29b-2-5p mimic. Forty-eight hours later, cell cycle arrest (a) and apoptosis (b) were analyzed by flow cytometry. The error line represents the mean ± SD, *P < 0.05. Forty-eight hours later, whole cell lysate was used for the Western blotting analysis. Cyclin D1, Bcl-2, Bax, and GAPDH were detected with their respective antibodies; n = 3 (c). Data are presented as mean ± SD (n = 3)