Fig. 4

MAP3K7 depletion does not inhibit NF-κB activation after stimulation of T-ALL cell lines with TNF-α. Six days after transduction with anti-MAP3K7 shRNA, T-ALL cell lines were stimulated with TNF-α (10 ng/μl) for 30 min and whole cell lysates were analyzed by Western blotting. a Stimulation of non-transduced T-ALL cells results in degradation of IκB. b MAP3K7 depletion does not prevent degradation of IκB after stimulation with TNF-α. c Treatment with anti-MAP3K7 shRNA did not consistently change expression patterns of NF-κB target genes in T-ALL cell lines. Six days after transduction with anti-MAP3K7 shRNA, total RNA was extracted, cDNA was synthesized and MAP3K7 mRNA expression quantified by qRT-PCR. HPRT1 was used as internal control. Mean values and SE of expression levels are given (n = 3). CCRF-CEM carries a PTEN deletion and does not express PTEN [62]