Fig. 4

Signaling pathways targeted by rFMW/GFP in ATC cell lines. a THJ-16 T and THJ-29 T cells were infected with rFMW/GFP for 6, 12 and 24 h. Protein levels of p-p38, total p38, p-Erk1/2, total Erk1/2, p-JNK and total JNK were analyzed by immunoblotting (IB). β-actin was used as a control for equal loading. b THJ-16 T and THJ-29 T cells were infected with vehicle or 10 MOI rFMW/GFP following pre-treatment with the p38 MAPK inhibitor, SB203580 (10 μM). Cell growth inhibition was determined using the MTT assay. Data are presented as the mean ± SEM, 0.001 < **p < 0.005. c Protein expression of total and cleaved caspase-3 and PARP was examined by IB