Fig. 4

Determination of apoptosis in RCC4 cells. In a group of cells with induced apoptosis (AIK), percentage of apoptotic cells increased significantly compared to control, untreated cells (cont). Blocking with CBS and CSE blockers AOAA and PGG (a) revealed prevention of apoptosis induction. When CBS (b), CSE (c) or MPST (d) were silenced prior the apoptosis induction, apoptosis was not induced. Effectivity of silencing of CBS was ̴ 60%, CSE ̴ 70% and MPST ̴ 50%, as determined by the gene expression. Representative gels are shown in section (e), where scr is scrambled siRNA. Results are displayed as mean ± S.E.M. and represent an average of 6 parallel from two independent cultivations. Statistical significance compared to control represents *** - p < 0.0001 and statistical significance compared to AIK treated cells represents +++ − p < 0.0001