Fig. 6

p52 supports viability and spheroid formation in anchorage resistant LA environment. a) Western blot and quantification demonstrating increased p52 protein levels and decreased activated MEK levels in LA relative to HA conditions. b) Loss of p52 or IKKε alone had no effect on viability in anchorage supportive conditions or anchorage resistant conditions after 48 h culture. Loss of both IKKε and p52 significantly reduced survival in the anchorage resistant conditions. Statistical analysis: ** indicates condition significantly different when compared to all HA and LA conditions, one-way ANOVA, post hoc-Tukey. c) Pharmacological inhibition of MEK significantly reduced survival in HA conditions and in the LA conditions when IKKε is also absent. Statistical analysis: * indicates condition significantly different from shNeg with vehicle in LA conditions; # indicates condition significantly different from shNeg with vehicle HA conditions, one-way ANOVA, post hoc-Tukey. d-e) Spheroid formation efficiency at 72 h under LA conditions is supported by p52, but not MEK. Statistical analysis: * indicates condition significantly different from neg shRNA, one-way ANOVA, post hoc-Tukey