Fig. 4

RSV induces time- and dose-dependent autophagy in HL-60 cells. a HL-60 cells were treated with different concentrations of RSV for 24 h, or treated with 50 μM RSV for different times, and western blot analysis was used for analyzing the expression of autophagy-related proteins by using the specific antibodies as indicated in figure. β-Actin was used as a loading control. b HL-60 cells were treated with different concentrations of RSV for 24 h, or treated with 50 μM RSV for different times, then semi-quantitative PCR was used for detecting the expression of autophagy-related genes Beclin-1 and P62. GAPDH was used as a control. c HL60 cells were treated with different doses of RSV for different times as indicated, then immunofluorescence was used for analyzing the expression of the autophagy marker, LC3. Data are presented as mean ± S.D. of three independent experiments in duplicates. *p < 0.05, vs. control