Fig. 1
From: Aggregation of lipid rafts activates c-met and c-Src in non-small cell lung cancer cells
Lipid rafts were separated by a sucrose density gradient centrifugation procedure, and immunoblotting was performed for c-Met, p-c-Met, c-Src, p-c-Src and flotillin-1. Blots are representative of at least three independent experiments. Fractions 2–6 were determined to be lipid raft fractions due to the presence of the lipid raft-specific protein flotillin-1. c-Met was mainly distributed in fractions 1 and 3–8; p-c-Met was mainly distributed in fractions 2–8; c-Src was mainly distributed in fractions 1–8; and p-c-Src was mainly distributed in fractions 2–6 and 10