Fig. 10

PI3K/Akt and MAPK/ERK1/2 pathways activation profile of RCC cells. a Dual pathway (PI3K and MAPK) profile of 786-O cells treated with agonist WIN-55 (0–25 μM). b Dual pathway (PI3K and MAPK) profile of ACHN cells treated with agonist WIN-55 (0–25 μM). ACHN and 786-O cells were found positive for Phospho-ERK1/2 (MAPK/ERK1/2) but negative for Phospho-Akt (PI3K/Akt). The dot plots showing WIN-55 treatment does not activate/inhibit PI3K and MAPK pathways even in higher concentrations (10–25 μM) as compared to the not treated control (0 μM, first column in Fig. a and b). In each fig. a and b dot blots in second and third raw represents cells single stained with Phospho-Akt (PI3K/Akt) and Phospho-ERK1/2 (MAPK/ERK1/2), respectively. Dot blots in fourth raw in each fig. (a and b) represents cells double stained with Phospho-Akt (PI3K/Akt) and Phospho-ERK1/2 (MAPK/ERK1/2)