Mutation allele frequency threshold does not affect prognostic analysis using next-generation sequencing in oral squamous cell carcinoma

Table 3 Correlation analysis between non-synonymous mutations of all targeted genes of CAGE and baseline characteristics in the 46 patients with oral squamous cell carcinoma

Characteristics	Total patients N = 46	Non-synonymous mutations		P value*
	Total patients N = 46	+	–
	n (%)	n (%)	n (%)
Gender
Male	12 (26.1)	10 (25.0)	2 (33.3)	0.644
Female	34 (73.9)	30 (75.0)	4 (66.7)	0.644
Age (years)
< 60	27 (58.7)	23 (57.5)	4 (66.7)	1.000
≥ 60	19 (41.3)	17 (42.5)	2 (33.3)	1.000
Site
Tongue	19(41.3)	18(45.0)	1(16.7)	0.182
Buccal	6(13.0)	6(15.0)	0(0.0)
Gingiva	6(13.0)	5(12.5)	1(16.7)
Floor of mouth	3(6.5)	2(5.0)	1(16.7)
Palate	9(19.6)	6 (15.0)	3 (50.0)
Retromolar trigone	3(6.5)	3(7.5)	0(0.0)
Clinical T stage
T1/T2	13(28.3)	11(27.5)	2(33.3)	1.000
T3/T4	33 (71.7)	29(72.5)	4(66.7)	1.000
Clinical N stage
N0	14(30.4)	11(27.5)	3 (50.0)	0.633
N1	12(26.1)	11(27.5)	1(16.7)
N2	20(43.5)	18(45.0)	2(33.3)
Clinical stage
III	22(47.8)	19(47.5)	3 (50.0)	1.000
IVA	24(52.2)	21(52.5)	3 (50.0)	1.000
Pathological differentiation grade
Well	13(28.3)	11(27.5)	2(33.3)	1.000
Moderately/Poorly	33(71.7)	29(72.5)	4(66.7)	1.000
Smoking status^a
Current/former	19(41.3)	17(42.5)	2(33.3)	1.000
Never	27(58.7)	23 (57.5)	4(66.7)	1.000
Alcohol use^b
Positive	24(52.2)	22(55.0)	2(33.3)	0.405
Negative	22(47.8)	18(45.0)	4(66.7)	0.405

*P value from the chi-square test was reported to compare the difference between the patients with and without non-synonymous mutation of targeted genes based on different baseline characteristics
^aFormer/current smokers defined as at least a one pack-year history of smoking
^bPositive alcohol use was defined as current alcohol use of more than one drink per day for 1 year (12 oz of beer with 5% alcohol, or 5 oz of wine with 12–15% alcohol, or one ounce of liquor with 45–60% alcohol). All other patients were classified as negative alcohol use
Note: No significant correlation was found between the non-synonymous mutant status of all targeted genes of CAGE and baseline characteristics in the 46 patients with oral squamous cell carcinoma

ISSN: 1471-2407