Fig. 2
From: Antiproliferative and apoptotic effect of LY2090314, a GSK-3 inhibitor, in neuroblastoma in vitro
LY2090314 treatment reduced the ability of forming colonies and also decreased the cell confluency in NB cell lines. a Colony Forming Unit (CFU) assay performed using NGP, SK-N-AS, and SH-SY-5Y cells, and increasing concentrations of LY2090314 compared to DMSO control. Inhibition by LY2090314 caused a decrease in NB cells ability to form colonies. b NGP, SK-N-AS, SH-SY-5Y cells treated with LY2090314 and cell proliferation were monitored in real time. The cells were imaged and the cell confluency was calculated using IncuCyte 2011A software. The changes in cell confluence are used as surrogate markers of cellular proliferation. As seen in this figure, LY2090314 effectively inhibited cell growth by decreasing cell confluency. Experiments were repeated three times and the best are shown here