Fig. 5

ANGPTL4 promotes monocyte proliferation and enhances osteoclast differentiation (a) Proliferation of monocytes and (b) formation of TRAP positive osteoclasts was increased by 10% conditioned media collected from ANGPTL4 over-expressing cells. (c) MG63-A4 conditioned medium caused elevated bone resorption of mature osteoclasts. (d, e) Quantification of the number of TRAP-positive multi-nucleated osteoclasts formed following treatment with different concentrations (50, 100, 200 ng/ml) of flANGPTL4, cANGPTL4 and nANGPTL4. *p < 0.05