Fig. 3

SETDB1 promoted cell metastasis both in vivo and vitro in HCC. a and b Transwell and Boyden assays indicate SETDB1 could enhance cell migration and invasion. The invasive cells were stained and counted under microscope at 24–30 h after reseeding. Original magnification, × 400. ** P<0.01, as compared LV-SET with WT and LV-con groups, or LV-SET with WT and LV-shcon groups. c SETDB1 significantly promoted HCC tumorigenicity in vivo as demonstrated by an orthotopic tumor implantation experiment in nude mice. d SETDB1 increased lung metastasis in an orthotopic tumor implantation model in nude mice. Hematoxylin and eosin staining confirming the formation of HCC tumor foci in the lungs. e SETDB1 modulates EMT-related genes expression