Skip to main content
Fig. 5 | BMC Cancer

Fig. 5

From: Sanguinarine triggers intrinsic apoptosis to suppress colorectal cancer growth through disassociation between STRAP and MELK

Fig. 5

Sanguinarine induced apoptosis of CRC cells by inhibition of the interaction of MELK and STRAP. a SW480 cells were treated with sanguinarine for 12 h. STRAP and MELK mRNA relative level was detected by qRT-PCR. b Western blotting analysis of MELK, STRAP, p-MELK and p-STRAP in SW480 cells after treatment of 2.4 μM sanguinarine for specified times. c The in vitro kinase activity of MELK was analyzed by using HTRF® KinEASE™-STK kit. d The interaction between MELK and STRAP was analyzed by Co-IP. Cells were lysed and immunoprecipitated using an anti-MELK or STRAP antibody. The precipitated proteins were analyzed using westernblotting. Data presented are showed as means ± SD from three independent experiments. # p < 0.01 indicates significant difference

Back to article page

BMC Cancer

ISSN: 1471-2407

Contact us