Fig. 2

Time-dependent analysis of enzymes involved in ceramide synthesis in GT3 treated BxPC3, Panc 1 and HPDE-E6E7 cells. Pancreatic cancer cells BxPC3(a-f), Panc 1 (g-l), and non-cancerous ductal pancreatic cells HPDE-E6E7 (m-r) were treated with GT3 at a concentration of 40 μM or dissolution vehicle as a control for different time periods (2, 4, 6 h). Cell lysates were analyzed by immunoblot. Membranes were incubated with antibodies against SPT, CERS6, and  DEGS1. The membranes were reprobed with actin as a loading control (located below each set of enzyme immunoblots, as in Figure 1). For quantification band densities from the treatment conditions identified by the lane labels, were calculated as percentages of the value for the treated, untreated cells (100%). Data shown of representative experiments, (mean ± SE, n = 3) *p<0.05, **p<0.01, significant difference between control and GT3 treated cells analyzed at different time points