Fig. 6

Galectin-3 affects lung cancer cell proliferation and migration through TLR4/NF-κB/NEAT1 (a) A549 and H1299 cells were transfected with Gal-3 in the presence or absence of CLI-095(50 nmol/L for 4 h); the protein levels of TLR4, MyD88 and p-p65 were determined using Immunoblotting assays. b The mRNA expression of NEAT1 was determined using real-time PCR assays. c-d The proliferation of lung cancer cell was determined using MTT and BrdU assays. e The migration of lung cancer cell was determined using Transwell assays. The data are presented as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, compared to control group; #P < 0.05, ##P < 0.01, Gal-3 group compared to Gal-3 + CLI-095 group