Fig. 2

GOLPH3 depletion inhibited the HCC cells proliferation and promoted the HCC cells apoptosis; a The relative expression of GOLPH3 mRNA in MHCC97L and MHCC97H cells transfected with si-GOLPH3 for 48 h, 96 h and 144 h. b The relative expression of GOLPH3 protein in MHCC97L and MHCC97H cells transfected with si-GOLPH3 for 48 h, 96 h, and 144 h. And si-Ct, si-GO represented si-Ctrl, si-GOLPH3 respectively. c MTT assay of HCC cells transfected with si-GOLPH3 for 48 h, 96 h and 144 h. d Flow cytometry for Annexin V-fluorescein isothiocyanate (FITC)-propidium iodide (PI) staining of the HCC cells treated with si-GOLPH3 for different time. e Hoechst 33,342 staining of HCC cells transfected with si-GOLPH3 after 144 h. The magnification of images was 400×. f Caspase3/7 activity assay of the HCC cells after transfected with si-GOLPH3 for 144 h. All experiments were performed in triplicate with at least three independent experiments. Data was presented as the mean ± SD. P-values were calculated using Student’s t-test, * P < 0.05, ** P < 0.01, *** P < 0.001, significant difference compared with the si-Ctrl cells in the same time