Fig. 5
From: The role of vimentin in the tumor marker Nup88-dependent multinucleated phenotype
Increased expression of phospho-vimentin in Nup88 overexpressing cells. Phospho-vimentin expression was analyzed in HeLa cells overexpressing GFP and Nup88-GFP by immunoblotting using antibodies against pan-vimentin, phospho-vimentin (pS83), GFP and α-tubulin (a). Relative expression of phospho vimentin in cells expressing GFP and Nup88-GFP (b). Data were collected from three independent experiments including the result shown in Fig. 5a (b). Cells in mitosis were monitored by an immunofluorescence assay. HeLa cells overexpressing GFP or GFP-tagged Nup88 were fixed and stained with antibodies against phospho-histone H3 (pH 3) to distinguish mitotic cells from interphase cells. Data were collected from six independent experiments. Error bars indicate ± SD (b and c). N. S. means not significant (c)