Fig. 4
From: The role of vimentin in the tumor marker Nup88-dependent multinucleated phenotype
Depletion of vimentin by siRNA transfection suppresses the formation of multinuclei induced by Nup88 overexpression. HeLa cell lines treated with control siRNA (siCont) or vimentin-specific siRNA (siVim) were overexpressed with GFP and GFP-tagged Nup88 in the presence of DOX for 2 days. Depletion of vimentin was confirmed by immunoblotting (a) and immunofluorescence (b). Multinucleated cells were counted in images obtained from the indirect immunofluorescence assay. Data were collected from at least four independent experiments. Total cell number analyzed in the experiment for siCont-treated GFP expressing cells, siCont-treated Nup88-GFP expressing cells, siVim-treated GFP expressing cells, and siVim-treated Nup88-GFP expressing cells were 1296, 1156, 1377 and 1284 cells, respectively (c). White arrowheads indicate multinuclei (binuclei) (b). Error bars indicate ± SD (c)