Fig. 3From: The role of vimentin in the tumor marker Nup88-dependent multinucleated phenotypeAnalysis of colocalization of overexpressed Nup88 with vimentin. HeLa cell lines overexpressing GFP and GFP-tagged Nup88 were fixed with 4% paraformaldehyde and permeabilized with 0.5% Triton X-100. GFP constructs, vimentin, and nuclei were stained with rabbit anti-GFP antibody (green), mouse anti-vimentin antibody (red), and Hoechst 33,342 (blue), respectivelyBack to article page