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Fig. 3 | BMC Cancer

Fig. 3

From: Chronophin regulates active vitamin B6 levels and transcriptomic features of glioblastoma cell lines cultured under non-adherent, serum-free conditions

Fig. 3

Establishment of CIN knockdown cell lines and cell proliferation analyzes. a and b Real-time PCR of CIN, c-Myc and NES in NCH644 (a) and NCH421k (b). While CIN levels are significantly reduced by two different CIN targeting shRNAs in NCH644 and NCH421k (p < 0.01), the mRNA levels of the glioblastoma stem cell markers c-Myc and NES are not affected. c and d Representative examples of CIN western blots in NCH644 (c) and NCH421k (d). The knockdown of CIN was efficient in both NCH644 and NCH421k. Alpha-tubulin was used as loading control. e and f Proliferation assay of shRNA knockdown cell lines in NCH644 e and NCH421k f. There are no significant differences between control and CIN shRNA cells (repeated measures two-way ANOVA followed by bonferroni corrected post-hoc tests)

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BMC Cancer

ISSN: 1471-2407

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