Fig. 6
From: Development of a computational promoter with highly efficient expression in tumors
Expression of different transcription factors in tumors of different sizes. a Immunohistochemical (IHC) staining of HIF-1α, NF-κB or CREB in different-sized B16F10 tumors. The tissue sections of tumors were probed with primary and HRP-conjugated antibodies, developed and photographed under a microscope (Scale bar: 100 μm, 200×). According to the levels of brown-colored staining in the tumor tissue sections, the expression levels of the TFs were quantified and calculated as protein expression scores. The protein expression scores for the groups with different tumor sizes (a: 50 mm3, b: 100 mm3, c: 250 mm3, d: 500 mm3 and e: 1000 mm3) were calculated and are displayed for HIF-1α, NF-κB or CREB. Statistically significant differences were determined by t-test, and the p values were presented as the tested group compared with the control group (*: p < 0.05). b After intra-tumor transfections with pCMV-hrGFP or pD5-hrGFP in different-sized B16F10 tumors for 7 days, the green fluorescent protein levels in the B16F10 tumors were observed under a fluorescence microscope (Scale bar: 100 μm, 200×) and photographed. The GFP expression scores were estimated and calculated according to the different tumor sizes (a: 50 mm3, b: 100 mm3, c: 250 mm3, d: 500 mm3 and e: 1000 mm3). Statistical analysis of the average score of green fluorescent protein expression in B16F10 tumors was performed by t-test (*p < 0.05). c After intra-muscle transfection with pCMV-hrGFP or pD5-hrGFP for 7 days, the green fluorescent protein levels in muscles were observed under a fluorescence microscope (Scale bar: 100 μm, 200×) and photographed. The GFP expression scores were estimated and calculated. Statistical analysis of the average green fluorescent protein expression score in the muscles was performed by t-test (*p < 0.05)