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Fig. 3 | BMC Cancer

Fig. 3

From: Cancer-derived exosomes from HER2-positive cancer cells carry trastuzumab-emtansine into cancer cells leading to growth inhibition and caspase activation

Fig. 3

T-DM1 carried on Type A exosomes may bind to cells and internalize. Confocal microscopy images of HER2-positive SKBR-3 breast cancer cells treated with Type A exosomes or unbound T-DM1. T-DM1 was stained with Alexa 488-goat anti-human-IgG (A488-GAHIG) and the nuclei with DAPI. No T-DM1 is detected in SKBR-3 cells when treated with MCF-7 exosomes treated with T-DM1 (ac) or when treated with SKBR-3 exosomes treated with PBS (df) (negative controls). Treatment of SKBR-3 cells with unbound T-DM1 (positive control) resulted in strong cell membrane staining (gi) and internalization of T-DM1 (insert j). Treatment of SKBR-3 cells with SNU-216 exosomes treated with T-DM1 (km) or with SKBR-3 exosomes treated with T-DM1 (oq) resulted in cell membrane staining, and internalization of some T-DM1 (inserts n and r, respectively). The images were taken with identical microscope settings

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