Fig. 3
From: NKX2.5 is expressed in papillary thyroid carcinomas and regulates differentiation in thyroid cells
Overexpression of NKX2.5 reduces thyroid differentiation markers and iodide uptake in PCCL3 cells: PCCL3 cells were transfected with empty vector (pcDNA3.1) or with the plasmid encoding for NKX2.5 (pNKX2.5) and, 24 h later, we have evaluated: (a) TSH receptor (TSHR), (b) Thyroperoxidase (TPO) and (c) Sodium-iodide symporter (NIS) mRNA levels, besides (d) iodide uptake (for RT-PCR, n = 7 per condition and for iodide uptake assay, n = 9 per condition; * p < 0.05 vs. pcDNA3.1, ** p < 0.01 vs. pcDNA3.1, ***p < 0.001 vs. pcDNA3.1). Data are expressed as relative to control (empty vector - pcDNA3.1). TSHR – thyrotropin receptor; TPO – thyroperoxidase; NIS – sodium-iodide symporter