Fig. 3

Effects of PCNP on the migration and invasion of human neuroblastoma cells. a The effect of PCNP on cell migration was measured by wound healing assay; original magnification 100 ×. b Soft agar assay was performed to examine the anchorage-independent survival of cells; original magnification 100 ×. c The migration rates of SH-SY5Y and SK-N-SH cells were calculated by the formula shown above. d The number of colonies was calculated. e Transwell assay was performed to assess the migration of SH-SY5Y and SK-N-SH cells; original magnification 200 ×. f Transwell assay was performed to assess the invasion of SH-SY5Y and SK-N-SH cells; original magnification 200 ×. g The numbers of the migrated cells were calculated. h The numbers of the invasive cells were calculated. Data are presented as mean ± SEM of three independent experiments; *P < 0.05, **P < 0.01 compared with the Mock group; ^#P < 0.05, ^##P < 0.01 compared with the sh-Scb group