Reduction of MHC-I expression limits T-lymphocyte-mediated killing of Cancer-initiating cells

BMC Cancer

Table 1 Sphere-forming capacity of selected human lung cancer cell lines

Cell line	Sphere-forming capacity^a	Long-term proliferation assay^b	Fold-expansion^c	Stem cell gene expression^d
Cell line	Sphere-forming capacity^a	Long-term proliferation assay^b	Fold-expansion^c	OCT4	NANOG	SOX2
A549	+	+	1.2 ± 0.3 (n = 15)	45 ± 10	112 ± 33	2 ± 0.4
H460	+	+	3 ± 2.1 (n = 24)	38 ± 10	55 ± 7	89 ± 36
H1299	+	+	1.3 ± 0.6 (n = 24)	115 ± 35	97 ± 17	79 ± 19
Calu-6	+	–	1.1 ± 0.9 (n = 12)	NA	NA	NA
H520	+	–	1 ± 1 (n = 20)	NA	NA	NA
H522	+	–	0.6 ± 0.6 (n = 17)	NA	NA	NA

^aAbility to form three-dimensional multicellular spheroids in tumorsphere culture. ^bMean fold-expansion > 1 and ability to culture > 3 passages. ^cFold-expansion average ± standard deviation. Fold-expansion is calculated over multiple passages. ^dGene expression fold-difference assessed by qRT-PCR of CICs (n = 2) normalized to GAPDH and standardized to matched non-CIC (n = 3) expression ±standard deviation, P = 0.005 for all. NA = Not applicable

ISSN: 1471-2407