Skip to main content

Table 1 Sphere-forming capacity of selected human lung cancer cell lines

From: Reduction of MHC-I expression limits T-lymphocyte-mediated killing of Cancer-initiating cells

Cell line Sphere-forming capacitya Long-term proliferation assayb Fold-expansionc Stem cell gene expressiond
OCT4 NANOG SOX2
A549 + + 1.2 ± 0.3
(n = 15)
45 ± 10 112 ± 33 2 ± 0.4
H460 + + 3 ± 2.1
(n = 24)
38 ± 10 55 ± 7 89 ± 36
H1299 + + 1.3 ± 0.6
(n = 24)
115 ± 35 97 ± 17 79 ± 19
Calu-6 + 1.1 ± 0.9
(n = 12)
NA NA NA
H520 + 1 ± 1
(n = 20)
NA NA NA
H522 + 0.6 ± 0.6
(n = 17)
NA NA NA
  1. aAbility to form three-dimensional multicellular spheroids in tumorsphere culture. bMean fold-expansion > 1 and ability to culture > 3 passages. cFold-expansion average ± standard deviation. Fold-expansion is calculated over multiple passages. dGene expression fold-difference assessed by qRT-PCR of CICs (n = 2) normalized to GAPDH and standardized to matched non-CIC (n = 3) expression ±standard deviation, P = 0.005 for all. NA = Not applicable