Fig. 4
From: microRNA-451a regulates colorectal cancer proliferation in response to radiation
miR-451a targets genes involved in cell cycle and cellular stress responses. a miR-TRAP assay depicting enrichment of target mRNAs immunoprecipitated from HCT-116 cells co-transfected with a mutant RISC complex plasmid and a miR-451a mimic or a control miR mimic. Fold enrichment over pre-IP mRNAs is depicted. One of two independent experiments. b qRT-PCR of the miR-451a targets in HCT-116 cells at 24 h after transfection. c Western blot for EMSY and CAB39 in HCT-116 cells at 24 h and 48 h after transfection of miR-451a compared to control mimic or mock transfection. Right panels show quantitation of band intensity at 48 h from two independent experiments. d siRNA mediated silencing of EMSY and CAB39 phenocopies the miR-451a effects in HCT-116 proliferation. * P < 0.05, Student’s T-test