Skip to main content
Fig. 5 | BMC Cancer

Fig. 5

From: Putative cancer stem cells may be the key target to inhibit cancer cell repopulation between the intervals of chemoradiation in murine mesothelioma

Fig. 5

Mesothelioma stem cell-associated genes. a Screening strategy of mesothelioma stem cell-associated genes by comparing parental RN5 cells with no treatment (NoRx), with cisplatin (Cis), γ-ray radiotherapy (RT), and enriched mesothelioma stem cells (MSC); b Overall differentiation of gene expression determined by principal component assay (PCA) mapping; c Total number of genes with a greater than 2-fold change of up- or down-regulation; d Venn diagram showing the overlapping genes of the 3 comparisons as depicted in (a); e Heatmap of gene expression in the 4 groups (NoRx, Cis, RT and MSC) as screened in the Venn diagram, which most likely contains mesothelioma-associated stem cell genes; f Novel genes including Tnfsf18, Serpinb9b, Ly6a and Nppb are confirmed to be upregulated by RT-qPCR; g Known genes CD24, CD117, CD133 and uPAR (CD87) are upregulated, as well as after treatment with chemoradiation. The experiment was carried out twice. The ratio of each gene to the house-keeping gene GAPDH is presented as mean ± SD. * P < 0.05, **P < 0.01, ***P < 0.001

Back to article page