Fig. 5

HES1 knockdown inhibits SACC cell migration and invasion ability. a, b A photomicrograph of the scratch wounds made in the siRNA-transfected SACC cell layer showing that cellular motility was inhibited in HES1-silenced cells compared with control cells (a) and the width of injury line after transfection for 72 h compared with 0 h was quantificated (b). c Representative images of transwell chambers coated without (upper panel) or with (lower panel) Matrigel after siRNA transfection. d The number of cells that migrated through the uncoated filters (i.e., no Matrigel), which represents the migratory ability of the indicated SACC cells. e The number of cells that were able to pass through the filters precoated with Matrigel, which represents the invasive ability of the indicated SACC cells. The cell counts are presented as the mean number of cells per field from at least five randomly selected low-powered fields (200×) from three independent experiments (error bars, means ± SD)