Fig. 3

Converted RR cells share similar biochemical features with RR cells. a The Western blotting results showed the protein levels of active β-catenin, total β-catenin, p-MYC^S62, and MYC in RU and RR cells derived from SupM2 and Karpas 299 cells, with or without H₂O₂ re-challenge. b The qRT-PCR results suggested that the Sox2 downstream targets including WNT2B and BCL9 significantly increased in mRNA level in converted RR cells (upper panel) and RR cells re-challenged with H₂O₂ (lower panel), in comparison with their counterpart cells. c SRR2 probe pull-down assay was performed to evaluate the Sox2-SRR2 DNA binding ability. The results suggested that more Sox2 was pulled down by biotin-labeled SRR2 probe in converted RR cells and RR cells after H₂O₂ stimulation as compared to their native RU and RR cells. The input of the pull-down assay demonstrated that the Sox2 expression was not appreciably altered in this experiment. Imag J software was utilized to analyze the densitometry. Densitometry values of proteins of interest were all normalized to β-actin bands, and the densitometry value of protein of interest in RU cells was normalized as 1.0