Fig. 5

G-Rh2 downregulated protein expression levels of VEGF-C, MMP9 and MMP2 in NSCLC cells. a-c A549 and co-cultured A549 with RAW264.7 derived M2 cells were treated with different concentrations of G-Rh2 for 24 h. Cell lysates were harvested. Protein expression levels of VEGF-C, MMP9 and MMP2 were examined by western blot. β-actin was used as a loading control. d-f)Quantification of VEGF-C, MMP9, and MMP-2 bands through quantification software. * P < 0.05, **P < 0.01, compared with respective control. g A549 and co-cultured A549 with THP-1 derived M2 cells were treated with different concentrations of G-Rh2 for 24 h. Cell lysates were harvested. Protein expression levels of VEGF-C was examined by western blot. β-actin was used as a loading control. h Quantification of VEGF-C bands through quantification software. * P < 0.05, **P < 0.01, compared with respective control