Fig. 2

Macrophages promote the proliferation of Raji cells while Raji cells enhance B7-H1 expression in macrophages (a) Raji cells were cultured with or without macrophages for 24, 48 and 72 h, followed by the analysis of the cell proliferation of Raji cells by BrdU incorporation assay (*P < 0.05). b Raji cells were cultured with or without macrophages for 72 h, and the cell cycle distribution of Raji cells was analyzed by flow cytometry (*P < 0.05). c Macrophages were cultured with or without Raji cells for 72 h, followed by the analysis of iNOS, CD206, CD163 and CD204 by flow cytometry. M2 phenotypes (CD163 and CD204) increased in macrophages (*P < 0.05). d Macrophages were treated as c, and B7-H1 expression was assessed by flow cytometry (*P < 0.05). e Macrophages were treated as c, and B7-H1 expression was assessed by western blot