Fig. 1

MiR-186-5p expression in PCa serum and cell lines. a Relative expression of miR-186-5p was validated in two independent isolations of miRNA from PCa serum (n = 15) relative to non-cancerous controls (n = 5). Elevated levels of miR-186-5p were detected in the serum of patients diagnosed with PCa tumor stage I and III (p ≤ 0.0001), and IV (p = 0.0007) disease. b Relative miR-186-5p expression was measured in normal prostate epithelial cells (RWPE1, RWPE2), prostate carcinoma xenograft (22Rv1), bone (PC-3), lymph node (LNCaP), brain (DU145), and LNCaP –derived bone (C4-2B) metastatic PCa cells using qRT-PCR. MiR-186-5p was up-regulated in four metastatic PCa cell lines (LNCaP, MDA PCa-2b, PC-3, 22Rv1) relative to normal prostate epithelial RWPE1 cells (p < 0.01). PC-3 cells exhibited the highest expression among the other cell lines (p = 0.0002). Data analyses were based on 2–3 independent experiments and presented as log [mean fold change] and mean fold change ± standard deviation (S.D.). (** p-value < 0.01, *** p-value < 0.0007, **** p-value < 0.0001) and one-way ANOVA analysis (** p-value < 0.005)