Fig. 4

D5D-KD and DGLA supplementation enhance the efficacy of 5-FU on growth of MDA-MB 231 cells. a Colony formation assay of D5D-KD MDA-MB 231 cells or control siRNA transfected cells at 10 days with treatment of 5-FU (10 μM) or 5-FU + DGLA (100 μM) for 48 h. Note, D5D-KD and NC-si control cells without DGLA and 5-FU treatment (shown in Fig. 2) were used to calculate survival fractions; and b Cell apoptosis was examined via flow cytometry after D5D-KD MDA-MB 231 cells were treated with DGLA (100 μM), 5-FU (10 μM), or 5-FU + DGLA for 48 h, followed by Annexin V-FITC/PI double staining. Data represent as mean ± standard deviation (*: significant difference vs. control with p < 0.05; #: significant difference vs. 5-FU group with p < 0.05 from n ≥ 3)