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Fig. 2 | BMC Cancer

Fig. 2

From: Knockdown delta-5-desaturase in breast cancer cells that overexpress COX-2 results in inhibition of growth, migration and invasion via a dihomo-γ-linolenic acid peroxidation dependent mechanism

Fig. 2

D5D-KD and DGLA inhibit MDA-MB 231 cell growth and migration. a Western blot and protein expression rate of D5D and COX-2 in MDA-MB 231cells after D5D siRNA transfection (β-actin as loading control). Similar inhibition of D5D (~ 70%) was also observed in shRNA transfected MDA-MB-231 cells; b GC/MS quantification of 8-HOA from cell medium containing 1.0 × 106 siRNA D5D-KD MDA-MB 231 cells or control siRNA transfected cells after 100 μM DGLA treatment. Similar results were also observed in shRNA transfected cell lines vs. their controls (data not shown); c Colony formation of D5D-KD MDA-MB231 or control siRNA transfected cells 10 days after DGLA treatment (100 μM for 48 h); and d Wound healing assays and quantification of wound area of D5D-KD MDA-MB 231 cells upon DGLA (100 μM, 48 h) treatment vs. controls (without DGLA). Data represent as mean ± standard deviation (*: significant difference with p < 0.05 from n ≥ 3)

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BMC Cancer

ISSN: 1471-2407

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