Fig. 6

Delphinidin suppressed the mTOR signalling pathway and activated the AMPK signalling pathway in MDA-MB-453 cells. a The suppression of upstream proteins from the mTOR signalling pathway, including AKT, phospho-AKT, mTOR, and phospho-mTOR, after the administration of different concentrations of delphinidin for 48 h. b The suppression of downstream proteins from the mTOR signalling pathway, including eIF4e, phospho-eIF4e, p70S6K, and phospho-p70S6K, after the administration different concentrations of delphinidin for 48 h. c The phosphorylated protein/β-actin ratio in the mTOR signalling pathway. d The activity of upstream proteins in the AMPK signalling pathway, including LKB1, phospho-LKB1, AMPK and phospho-AMPK, after administration of different concentrations of delphinidin for 48 h. e The activity of downstream proteins from the AMPK signalling pathway including FOXO3a, phospho-FOXO3a, ULK1, and phospho-ULK1 after administration of different concentrations of delphinidin for 48 h. f The phosphorylated protein/β-actin ratio in the AMPK signalling pathway. Dp: Delphinidin. Data were obtained from three independent experiments. *P < 0.05 vs control group; ^#P < 0.05 vs Dp group