Fig. 4

Extl1 associated with hyper-DMR is down-regulated in the liver tumor tissues of C3H mice. a Schematic representation of the Extl1 locus. The position of TSS, DMR and the position assessed by bisulfite sequencing (BS) were indicated. The magnified view indicates 27 CpG positions in the DMR and predicted transcription factor binding sites. b The average methylation values of the CpG sites in normal and tumor tissues. The CpG sites with ≥10 reads on both strands are indicated. Statistical significance between normal and tumor tissue was analyzed by Student’s t-test. *, **, *** Significantly different at p < 0.05, 0.01 and 0.001, respectively. c Validation of RRBS data for normal and tumor liver tissues of C3H mice by bisulfite sequencing. Methylated and unmethylated cytosine are shown as ● and ○ respectively. d Validation of gene expression levels of Extl1 by real-time PCR in the normal (n = 6) and tumor tissues (n = 11) of C3H mice. The expression of Extl1 is normalized to the expression of rRNA. Statistical significance between the two groups was analyzed by the Student’s t-test. * Significantly different at P < 0.05