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Fig. 3 | BMC Cancer

Fig. 3

From: Regulation of CNKSR2 protein stability by the HECT E3 ubiquitin ligase Smurf2, and its role in breast cancer progression

Fig. 3

Co-localization of Smurf2 and CNKSR2 in HEK293 and MDA-MB-231 cell lines. a HEK293 cells were transfected with Flag-Smurf2, and stained with mouse anti-Flag and rabbit anti-CNKSR2, followed by FITC-conjugated goat anti-mouse and PE-conjugated goat anti-rabbit secondary antibodies. b Magnified view of several cells imaged in A. c Endogenous Smurf2 and CNKSR2 co-localizes in MDA-MB-231 cells stained with goat anti-Smurf2 and rabbit anti-CNKSR2, followed by FITC-conjugated rabbit anti-goat and PE-conjugated goat anti-rabbit secondary antibodies. d Magnified view of several cells imaged in (c). Arrows illustrates the presence of co-localization

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