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Fig. 1 | BMC Cancer

Fig. 1

From: Regulation of CNKSR2 protein stability by the HECT E3 ubiquitin ligase Smurf2, and its role in breast cancer progression

Fig. 1

Smurf2 interacts with CNKSR2. (a, b) CNKSR2 coimmunoprecipitates with endogenous Smurf2 but not Smurf1. Cell lysates were prepared from MDA-MB-231 cells and were immunoprecipitated (IP) with rabbit CNKSR2, rabbit Smurf2 or control antibodies. Precipitates were resolved by 4–10% SDS-PAGE followed by immunoblotting (IB) with rabbit Smurf2, rabbit Smurf1 and rabbit CNKSR2. c Protein levels of Smurf2, Smurf1, CNKSR2 and β-actin in 1% of the total cell lysate were also shown

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