Fig. 1From: Regulation of CNKSR2 protein stability by the HECT E3 ubiquitin ligase Smurf2, and its role in breast cancer progressionSmurf2 interacts with CNKSR2. (a, b) CNKSR2 coimmunoprecipitates with endogenous Smurf2 but not Smurf1. Cell lysates were prepared from MDA-MB-231 cells and were immunoprecipitated (IP) with rabbit CNKSR2, rabbit Smurf2 or control antibodies. Precipitates were resolved by 4–10% SDS-PAGE followed by immunoblotting (IB) with rabbit Smurf2, rabbit Smurf1 and rabbit CNKSR2. c Protein levels of Smurf2, Smurf1, CNKSR2 and β-actin in 1% of the total cell lysate were also shownBack to article page