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Table 3 Secondary antibodies used for double immunofluorescence labeling or immunohistochemistry

From: Stellate cells and mesenchymal stem cells in benign mammary stroma are associated with risk factors for breast cancer – an observational study

Secondary antibodies for dIF Product code Visualization Dilution Manufacturer
Donkey anti-mouse IgG (Alexa Fluor 488 conjugated) 715–546-150 ALDH1 1:200 JIR
Goat anti-rat IgM (Rhodamine-Red conjugated) 112–295-020 SSEA-3 1:200 JIR
Donkey anti-rabbit IgG (Rhodamine red conjugated) 711–296-152 Tryptase, Vinculin 1:200 JIR
Goat anti-rabbit IgG Alexa Fluor 488 conjugated A11070 Tryptase 1:200 Invitrogen
Secondary antibodies for IHC Product code Visualization Dilution Manufacturer
Goat anti-mouse IgG (HRP conjugated) K4007 ALDH1 Pre-diluted Dako/Agilent
Goat anti-rabbit IgG (HRP conjugated) K4011 Tryptase Pre-diluted Dako/Agilent
Goat anti-rat IgM (HRP conjugated) 112–036-075 SSEA-3 1:100 JIR
  1. Abbreviations: dIF double immunofluorescence, IHC immunohistochemical labeling, JIR Jackson ImmunoResearch (West Grove, PA, USA); Invitrogen, Invitrogen, Thermo Fisher Scientific (Waltham, MA, USA); Dako/Agilent, Dako, Agilent Technologies (Santa Clara, CA, USA)