Stellate cells and mesenchymal stem cells in benign mammary stroma are associated with risk factors for breast cancer – an observational study

Table 3 Secondary antibodies used for double immunofluorescence labeling or immunohistochemistry

Secondary antibodies for dIF	Product code	Visualization	Dilution	Manufacturer
Donkey anti-mouse IgG (Alexa Fluor 488 conjugated)	715–546-150	ALDH1	1:200	JIR
Goat anti-rat IgM (Rhodamine-Red conjugated)	112–295-020	SSEA-3	1:200	JIR
Donkey anti-rabbit IgG (Rhodamine red conjugated)	711–296-152	Tryptase, Vinculin	1:200	JIR
Goat anti-rabbit IgG Alexa Fluor 488 conjugated	A11070	Tryptase	1:200	Invitrogen
Secondary antibodies for IHC	Product code	Visualization	Dilution	Manufacturer
Goat anti-mouse IgG (HRP conjugated)	K4007	ALDH1	Pre-diluted	Dako/Agilent
Goat anti-rabbit IgG (HRP conjugated)	K4011	Tryptase	Pre-diluted	Dako/Agilent
Goat anti-rat IgM (HRP conjugated)	112–036-075	SSEA-3	1:100	JIR

Abbreviations: dIF double immunofluorescence, IHC immunohistochemical labeling, JIR Jackson ImmunoResearch (West Grove, PA, USA); Invitrogen, Invitrogen, Thermo Fisher Scientific (Waltham, MA, USA); Dako/Agilent, Dako, Agilent Technologies (Santa Clara, CA, USA)

ISSN: 1471-2407