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Table 2 Primary antibodies used for simultaneous visualization of two antigens by double immunofluorescence or single immunohistochemical labeling of individual antigens

From: Stellate cells and mesenchymal stem cells in benign mammary stroma are associated with risk factors for breast cancer – an observational study

Epitope Host species Product code Dilution IHC Dilution dIF Manufacturer
ALDH1 A1 Mouse (IgG) 611,194 1:1000 1:100 BD
SSEA-3 Rat (IgM) MC-631 1:100 1:50 DSHB
Tryptase Rabbit (IgG) Ab134931 1:2000 1:2000 Abcam
Vinculin Rabbit (IgG) SAB4503069   1:300 Sigma-Aldrich
  1. MC-631 (SSEA-3) was deposited to the DSHB by Solter, D. / Knowles, B.B
  2. Abbreviations: dIF double immunofluorescence, IHC immunohistochemical labeling, BD Becton Dickinson Bioscience (Franklin Lakes, NJ, USA), DSHB Developmental Studies Hybridoma Bank (University of Iowa, Iowa City, IA, USA); Abcam (Cambridge, UK); Sigma-Aldrich (St. Louis, MO, USA)