Stellate cells and mesenchymal stem cells in benign mammary stroma are associated with risk factors for breast cancer – an observational study

Table 2 Primary antibodies used for simultaneous visualization of two antigens by double immunofluorescence or single immunohistochemical labeling of individual antigens

Epitope	Host species	Product code	Dilution IHC	Dilution dIF	Manufacturer
ALDH1 A1	Mouse (IgG)	611,194	1:1000	1:100	BD
SSEA-3	Rat (IgM)	MC-631	1:100	1:50	DSHB
Tryptase	Rabbit (IgG)	Ab134931	1:2000	1:2000	Abcam
Vinculin	Rabbit (IgG)	SAB4503069		1:300	Sigma-Aldrich

MC-631 (SSEA-3) was deposited to the DSHB by Solter, D. / Knowles, B.B
Abbreviations: dIF double immunofluorescence, IHC immunohistochemical labeling, BD Becton Dickinson Bioscience (Franklin Lakes, NJ, USA), DSHB Developmental Studies Hybridoma Bank (University of Iowa, Iowa City, IA, USA); Abcam (Cambridge, UK); Sigma-Aldrich (St. Louis, MO, USA)