Fig. 3

Confocal images of double immunofluorescence labeling of ALDH1 and tryptase. The images are digital composites from one focal plane showing merged channels with dIF-labeled ALDH1 (green) and tryptase (red), together with nuclear staining with DAPI (blue). a Image illustrating two ALDH1– SSEA+ round/oval cells (strong red) above scattered ALDH1+ SSEA– spindle-shaped cells (green). Scale bar 10 μm. b Image showing an area of tissue containing one large ALDH1+ tryptase+ double-labeled r/o cell (arrow). The ALDH1 labeling (green) to the right of this cell represents parts of ALDH1+ cytoplasm from different cell types. Double labeling appears as yellow. Scale bar 5 μm