Table 1 Primers used for RT_PCR of human GABA receptor subunits and GADs
Target (accession no.) |  | Primer sequence (5,_3’) | Position | Length (bp) |
|---|---|---|---|---|
GABAAα1 (NM_000806) | F | GGAATTGT C CAGT CAAGTACAGG | 1150–1172 | 532 |
R | TTGTTTCGGGCTTGACCTC | 1663–1681 | ||
GABAAα2 (NM_000807> | F | GCTTATGCAGTGGCTGTTGC | 1411–1430 | 257 |
R | GGACTGAC C C CTAATACAGGTTC | 1645–1667 | ||
GABAAα3 (NM_000808) | F | C CAC CT AT C C CAT CAAC CTG | 1442-1461 | 261 |
R | TGCCCTTGATAGCTGACTCC | 1683–1702 | ||
GABAAα4 (NM_000809) | F | AGACATCAAA GCCCCCTCAG | 2044–2063 | 307 |
R | GGAGAAGCAGATGGAAGTGC | 2331–2350 | ||
GABAAα5 (NM_000810) | F | CGCTTTTACAACTGGGAAGATG | 1657–1678 | 277 |
R | GAGTGTGGCCGGTTATTTTG | 1914-1933 | ||
GABAAα6 (NM_000811) | F | CCCACCCACA GTGACAATAT C | 1367–1387 | 331 |
R | TTCAACACTGCTACTGACTTCC | 1676–1697 | ||
GABAAβ1 (NM_000812) | F | AATCCGGAATGAGACGAGTG | 1493–1512 | 326 |
R | GGAAC CATTAGAACAGAC CT CAG | 1796–1818 | ||
GABAAβ2 (A28108) | F | TGCCAACAATGAGAAGATGC | 1259–1278 | 401 |
R | AGT GGGAGGC CAT GTTTTAG | 1640–1659 | ||
GABAAβ3 (NM_000814) | F | GACCGTTCAAAGAGCGAAAG | 1168-1187 | 233 |
R | CGTAGATGGGTCTTCTTGTGC | 1380-1400 | ||
GABAAγ1 (NM_173536) | F | TAAAGCCTCG ATGACTCCTG | 1274–1293 | 311 |
R | GACTTCTTTTGATTTTTGCTTATGG | 1560-1583 | ||
GABAAγ2 (NM_000816) | F | TTTGTCAGCAACCGGAAAC | 1433–1451 | 355 |
R | C CATATCAGTAAAACCCATAC CTC | 1764-1787 | ||
GABAAγ3 (NM_033223) | F | C CAAC CAC CAC GAAGAAGAC | 1305–1324 | 397 |
R | GCTCTTCACTCTTCACTCTGAGC | 1679–1701 | ||
GABAAδ (NM_000815) | F | ATTT CAACGC CGACTAC AGG | 1090–1109 | 300 |
R | GGGCGTAAATGTCAATGGTG | 1370–1389 | ||
GABAAε (NM_00496l) | F | GACAAAAG C C CAT G CTTCTC | 1144-1163 | 255 |
R | AAACGCTTGCACCACTCAC | 1380–1398 | ||
GABAAπ (U95367) | F | GTTTGAGCTT CGGAGGAATG | 861–880 | 201 |
R | AAGCAGTTGGTGTTGGGAAG | 1042–1061 | ||
GABAAθ(BD106470) | F | CCTCAGCCCACTCACTTCTC | 1278-1297 | 297 |
R | GCTTCTTGCACACCCTTCTC | 1555-1574 | ||
GABABR1a (AJ012185) | F | CAACGC CACCTCAGAAG | 69–85 | 234 |
R | GAGCAGATTCGGACACAG | 285-302 | ||
GABABR1a/b (AJ012186) | F | CTGAATCCTGCCAATACCC | 937–955 | 256 |
R | AGTTCATTGCCCGGTAGA | 1175-1192 | ||
GABABR2 (AJ012188) | F | GACCATCTCAGGAAAGACTC | 984–1003 | 235 |
R | GGTCTCGTTCATGGCATT | 1201-1218 | ||
GAD65 (NM_000818) | F | ATGC CT C CTACCT CTTT CAG | 1406–1425 | 218 |
R | CACCATCTCATATCCTTCTC | 1604-1623 | ||
GAD67 (NM_000817) | F | ACTGGCTGAATACCTCTATG | 2005–2024 | 318 |
R | CCCAGT CTTT CTATCTCCTC | 2303-2322 | ||
| Â | F: forward; R: reverse | Â |