Fig. 5

Brefeldin A inhibits MCF7 cancer cell clustering and calcein transfer. a Representative in-focus bright-field images are shown for each brefeldin A concentration and at the indicated time points. b Variation of the area occupied by MCF7 cells during the clustering assay with cells incubated with brefeldin A at the following concentration: 0.01 μM (n = 10), 0.1 μM (n = 17), 1 μM (n = 16) compared with untreated Control (n = 17). Results are the mean ± SD of 3 independent experiments (except for 0.01 μM: 2 independent experiments). Unpaired two-tailed t-tests at 2 h, ***p < 0.0005. c Transfer of calcein during clustering from donor positive cells to negative cells incubated or not with 0.01, 0.1, and 1 μM brefeldin A. The percentage of receiver newly positive cells is indicated. Results are the mean ± SD of 3 independent experiments (3 replicates per experiment). Unpaired two-tailed t-tests, at 5 h and 10 h *p < 0.05 and ***p < 0.0005