Fig. 5

Validation of microarray gene expression data by qPCR using selected genes. The fold change of selected genes were normalized to TBP in RT-qPCR (expression in iPSC-CRL-1831 (dark bars) and CSC-DLD1 (light bars) cells grown under 3D cell culture conditions compared to 2D monolayer). Each bar represents the mean and standard deviations of three independently performed experiments for RT-qPCR. Bars with no pattern represent fold change of the microarray experiment (where p-value < 0.05), while bars with pattern - RT-qPCR