Fig. 1

Cell proliferation regionalization in 3D spheroids. iPSC-CRL-1831 and CSC-DLD1 spheroids grown for seven days in the indicated conditions were incubated with EdU for 24 h prior to fixation and analysis. Representative confocal microscopy images of cryosections from spheroids. EdU detection was performed with Alexa FLuor® 488 (green). Nuclei were counterstained with DAPI (blue). Scale bar, 100 μm