Fig. 2

a Inhibition of TRPC5 by shRNA resulted in decreased TRPC5 protein expression (n = 4, *p < 0.05, Student’s t-test). b Up-regulated TRPC5 expression induces elevated [Ca²⁺] _i level in 5-Fu chemoresistant human CRC cells. The level of [Ca²⁺] _i in HCT-8/5-Fu/Scrambled cells was higher than in HCT-8 cells and dramatically decreased after the inhibition of TRPC5 by shRNA. (n = 4, *p < 0.05, one-way ANOVA) (Scrambled: scrambled shRNA). Scale bars, 20 μm