Fig. 5

Knockdown of HOXB3 and CDCA3 partially mimics the anti-leukemia activity by miR-375. a HL-60 and THP1 cells were transduced with special shRNA for HOXB3 or a control shRNA (sh-NC). HOXB3 and CDCA3 expressions were detected by western blot. b Viable cell number by the trypan-blue exclusion assay was counted in HL-60 and THP1 cells, which were transduced with special shRNA for HOXB3 or sh-NC for the indicated times. *P < 0.01 versus sh-NC. c Colony number was counted in HL-60 and THP1 cells, which were transduced with special shRNA for HOXB3 or sh-NC. *P < 0.01 versus sh-NC. d The protein expression of CDCA3 was detected in HL-60 and THP1 cells following transduction with special shRNA for CDCA3 or sh-NC. e Viable cell number by the trypan-blue exclusion assay was counted in HL-60 and THP1 cells infected with special shRNA for CDCA3 or sh-NC for the indicated time. *P < 0.01 versus sh-NC. f Colony number was counted in HL-60 and THP1 cells, which were transduced with special shRNA for CDCA3 or sh-NC. *P < 0.01 versus sh-NC