Fig. 2
The sensitivity of the LAMP assay using HPV LAMP primers to detect HPV 16 (a), 18 (b), 31 (c), and 35a (d). LAMP reactions were assessed by 2% agarose gel electrophoresis, visual assessment of precipitate formation in the reaction tube, and the presence (+) or absence (−) of spectrophotometer absorbance at 400 nm compared to negative controls. Lane M: 100 bp marker, lane N: negative control, lanes 1-7: 106 to 100 copies of 10-fold serial dilutions of purified plasmid DNA containing genomic inserts extracted from HPV 16, HPV 18, HPV 31, HPV 35a